Phytochemical Screening and Quantitative Analysis of Brown Algae Sargassum wightii Greville and Padina pavonica (L.) Thivy Collected from Mandapam, Tamil Nadu, India

ANUSIYA KUMARI M , Dr. Premalatha S , Dr. Aron Santhos Kumar Y

Sargassum wightii; Padina pavonica; brown algae; phytochemical screening; secondary metabolites; total phenolic content; total flavonoid content; Mandapam

Marine brown algae are well-recognized repositories of structurally diverse secondary metabolites with considerable pharmacological potential. The present study investigated the qualitative phytochemical composition and quantitative phenolic and flavonoid contents of petroleum ether, ethanol, and aqueous extracts of two ecologically important brown algae — Sargassum wightii Greville and Padina pavonica (L.) Thivy — freshly collected from the intertidal zone of Mandapam, Ramanathapuram District, Tamil Nadu, India. Shade-dried powdered material was subjected to sequential cold maceration in three solvents of ascending polarity, and the resulting crude extracts were screened for ten classes of secondary metabolites using standard colorimetric and precipitation methods (Harborne, 1998; Trease and Evans, 2002). Total phenolic content (TPC) and total flavonoid content (TFC) were determined spectrophotometrically by the Folin–Ciocalteu and aluminium chloride methods respectively, with all assays performed in triplicate. Sargassum wightii yielded the most chemically diverse profile: alkaloids, cardiac glycosides, phenols, flavonoids, tannins, steroids, terpenoids, proteins, and carbohydrates were detected, with the ethanol extract recording the highest TPC (18.42 ± 0.94 mg GAE/g dw) and TFC (11.63 ± 0.82 mg QE/g dw). Padina pavonica contained flavonoids, tannins, saponins, steroids, terpenoids, alkaloids, phenols, proteins, and carbohydrates across the three solvents, with ethanol again yielding the highest TPC (8.36 ± 0.62 mg GAE/g dw) and TFC (2.87 ± 0.31 mg QE/g dw). The distribution of compound classes across solvents of ascending polarity demonstrates that extraction solvent is a primary determinant of metabolite recovery. These findings establish the chemical foundation for subsequent bioactivity-focused studies on both species.

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"Phytochemical Screening and Quantitative Analysis of Brown Algae Sargassum wightii Greville and Padina pavonica (L.) Thivy Collected from Mandapam, Tamil Nadu, India", JETNR - JOURNAL OF EMERGING TRENDS AND NOVEL RESEARCH (www.JETNR.org), ISSN:2984-9276, Vol.4, Issue 6, page no.a41-a49, June-2026, Available :https://rjpn.org/JETNR/papers/JETNR2606008.pdf

Volume 4 Issue 6, June-2026

Pages : a41-a49

Paper Reg. ID: JETNR_234941

Published Paper Id: JETNR2606008

Downloads: 00078

Research Area: Life Sciences All

Country: COIMBATORE, Tamil Nadu, India

Published Paper PDF: https://rjpn.org/JETNR/papers/JETNR2606008

Published Paper URL: https://rjpn.org/JETNR/viewpaperforall?paper=JETNR2606008

ISSN: 2984-9276 | IMPACT FACTOR: 9.87 Calculated By Google Scholar | ESTD YEAR: 2023

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